Table 1.

Primer sets used for genotyping H. pylori by PCR.

Genes	Primer sequence (5′ à3′.)	PCR product (bp)	PCR conditions	References
glmM	AAGCTTTTAGGGGTGTTAGGGGTTT	294	93°C, 1 min; 55°C, 1 min; 72°C, 1 min (35 cycles)	10
	AAGCTTACTTTCTAACACTAACGC
vacA s1/s2	ATGGAAATACAACAAACACAC	259/286	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)	10
	CTGCTTGAATGCGCCAAAC
s1a	GTCAGCATCACACCGCAAC	190	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)	10
	CTGCTTGAATGCGCCAAAC
s1b	AGCGCCATACCGCAAGAG	187	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)	10
	CTGCTTGAATGCGCCAAAC
s1c	CTCTCGCTTTAGTGGGGYT	213	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)	10
	CTGCTTGAATGCGCCAAAC
m1/m2	CAATCTGTCCAATCAAGCGAG	567/642	94°C, 1 min; 52°C, 1 min; 72°C, 1 min (35 cycles)	10
	GCGTCAAAATAATTCCAAGG
cagA	ATAATGCTAAATTAGACAACTTGAGCGA	298	94°C, 1 min; 60°C, 1 min; 72°C, 1 min (45 cycles)	10
	TTAGAATAATCAACAAACATCACGCCAT
cagE	TTGAAAACTTCAAGGATAGGATAGAGC	508	94°C, 1 min; 53°C, 45 s; 72°C, 45 s (35 cycles)	10
	GCCTAGCGTAATATCACCATTACCC
iceA1	GTGTTTTTAACCAAAGTATC	247	95°C 1 min; 57°C, 1 s; 72°C, 1 min (35 cycles)	10
	CTATAGCCATTATCTTTGCA
iceA2	GTTGGGTATATCACAATTTAT	229	95°C 1 min; 57°C, 1 s; 72°C, 1 min (35 cycles)	10
	TTTCCCTATTTTCTAGTAGGT
babA2	CCAAACGAAACAAAAAGCGT	271	94°C, 1 min; 45°C, 1 min; 72°C, 1 min (30 cycles)	10
	GCTTGTGTAAAAGCCGTCGT
oipA	GTTTTTGATGCATGGGATTT	401	94°C, 1 min; 56°C, 1 min; 72°C, 1 min (35 cycles)	5
	GTGCATCTCTTATGGCTTT