Fig. 5.

Fructose induced oleate (C18:1) synthesis, acetyl-CoA formation and lactate release from adipocytes. Adipocytes were exposed to 0.1, 0.5, 1, 2.5, 5, 7.5 or 10 mM fructose in a medium containing a baseline amount of 5 mM glucose from the initiation of differentiation for 8 days (differentiating adipocytes) or 16 days (differentiated adipocytes). 10 % of fructose was supplied as [U-¹³C₆]-fructose for a period of 48 h before harvest. Intracellular oleate content in differentiating (a) and differentiated (b) adipocytes; ¹³C labeled intracellular acetyl-CoA in differentiating (c) and differentiated (d) adipocytes. ¹³C labeled lactate fraction as percent of total unlabeled lactate in the media in differentiating (e) adipocytes and in differentiated adipocytes (f), *p < 0.05 compared to the 0.1 mM fructose labeled