Fig. 4.

Semi-quantitative cDNA sequencing of eight RPE/retina tissue samples heterozygous for synonymous coding SNP rs17810398:C>T. The chromatograms of the variant nucleotide at rs17810398 flanked by ±3 bp are shown for isoforms 1 & 2, 3, and 4. Isoforms were specifically amplified by three different exon-spanning primer combinations. Genotypes of the four candidate variants are given above the chromatograms. The sample heterozygous for rs17810398 but homozygous for the non-risk alleles of rs6416986, rs17810816, and rs144087548 is highlighted in red