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. 2015 May;185(5):1436–1447. doi: 10.1016/j.ajpath.2015.01.031

Figure 1.

Figure 1

Targeted deletion of collagen V in flexor digitorum longus (FDL) tendons. A: Cre excision was targeted to tendons by using a Scleraxis promoter (Scx-Cre) in a double reporter (mTmG) mouse. Analysis of cross sections from postnatal day 4 (P4) limbs shows ubiquitous expression of red fluorescence (mT) in control mTmG mice. In contrast, Scx-Cre/mTmG mice show green fluorescence (mG) in the tendon, indicating targeted Cre excision. Other tissues show no Cre recombinase activity. DAPI (blue), nuclear localization. B: Col5a1 mRNA expression in the postnatal day 10 Col5a1Δten/Δten FDL is at background levels at real-time PCR. In contrast, expression in Col5a1flox/flox (Col5a1f/f) control mice is comparable with that in the wild-type mice. C: Postnatal day 10 FDLs are collagen V null in Col5a1Δten/Δten mice. Immunoblot analysis using antibodies against the alpha 1 chain of collagen V [a1(V)] and against β-actin as a loading control. Control Col5a1flox/flox and wild-type mice express comparable collagen V. D: Collagen V immunoreactivity (red) is present in postnatal day 30 control (Ctrl) FDLs but absent in Col5a1Δten/Δten mice. DAPI (blue), nuclear localization. Scale bars = 20 μm (A).