Fig. 4.

Method validation using live imaging of a zebrafish embryo. (A) Selected images from a movie of zebrafish epiboly. Scale bar: 200 μm. Each frame is in an arbitrary rotational orientation and the order of the frames has been shuffled. (B) Images from A after registration and ordering using vector diffusion maps. The real time of each frame is also indicated. (C) Correlation between the rank recovered using vector diffusion maps and the true rank. The rank correlation coefficient is 0.9954. The larger errors in the recovered ranks towards the beginning of the trajectory are due to slow cell movement within that time window.