A: Glomerular S100A8/A9 and macrophage CD68 expression in the accelerated nephrotoxic nephritis model in wild-type (WT) mice (n = 9). Number of cells per glomerular cross section was counted (for 25 glomeruli), mean of 3.53 (SD, 1.0) CD68-positive macrophages per glomerular section in WT animals, whereas there was a mean of 1.22 (SD, 0.6) S100A8/A9 cells per glomerular section. B: Graph shows the serum levels of S100A8/A9 in WT mice after nephrotoxic nephritis and in WT controls in which disease has not been induced. The serum level of S100A8/A9 is significantly higher in mice with nephrotoxic nephritis (P < 0.0005). GCS, glomerular cross section; NTN, nephrotoxic nephritis.