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Alveolar macrophage cell counts. Male rats were subjected to non-ventilate or low VT or high VT. After 4 h of the procedure, AM cells were isolated from the lung. Numbers of viable cells were determined by trypan blue staining and counted by a hemocytometer. Data are mean ± SD, n=10. #, P<0.001 vs. non-ventilated animals; $, P<0.001 vs. low VT; *, P>0.05 vs. non-ventilated animals.
