Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 4;212(5):793–807. doi: 10.1084/jem.20141849

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Carr et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — LEF1 promoted development of iNKT cells that produce IL-4 or IL-4 plus IFNγ. (A) Expression of IL-4 and IFNγ in PLZF^hi and PLZF^lo iNKT cells was determined by flow cytometry, after a 4-h in vitro stimulation of thymocytes with PMA and ionomycin. (B) Expression of IL-4, IFNγ, or IL-17A in thymic Tetr⁺TCRβ⁺ iNKT cells from control and Lef1^Δ/Δ mice after a 4-h in vitro stimulation of thymocytes with PMA and ionomycin. Plots are representative of four to six independent experiments. (C) The mean percentage of control and Lef1^Δ/Δ iNKT cells that expressed IL-4, IFNγ, IL-4 plus IFNγ, or IL-17A. Data were averaged from six independent experiments. (D) Expression of CD8α and EOMES in TCRβ⁺CD8α⁺ thymocytes was determined by flow cytometry. (E) The mean percentage of EOMES⁺ cells among TCRβ⁺CD8α⁺ thymocytes from four independent experiments. All error bars represent SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.