Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 4;212(5):793–807. doi: 10.1084/jem.20141849

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Carr et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — LEF1 was required for proper expression of GATA3 in CD4⁺ iNKT cells. (A) Expression of Gata3 mRNA relative to Hprt in sorted ST1, ST2, and ST3 iNKT cells from control and Lef1^Δ/Δ mice was determined by qRT-PCR. Data were combined from three independent experiments. (B) GATA3 versus CD4 expression in ST1, ST2, and ST3 thymic iNKT cells from control and Lef1^Δ/Δ mice, as determined by flow cytometry. (C and D) MFI for GATA3 in ST1, ST2, and ST3 iNKT cells (C) or in CD4⁺ and CD4⁻ iNKT cells (D) from the thymus of control and Lef1^Δ/Δ mice. (E) GATA3 versus CD4 expression in iNKT1 (PLZF^loTBET⁺) and iNKT2 (PLZF^hiTBET⁻) cells from control and Lef1^Δ/Δ mice, as determined by flow cytometry. Data are representative of four independent experiments. (F) MFI for GATA3 in thymic CD4⁺ iNKT2 and CD4⁺ iNKT1 cells from the indicated mouse strains. (C, D, and F) Each circle represents one mouse (paired Student’s t test: *, P < 0.05; **, P < 0.01). (G) LEF1 chromatin binding was determined by a-LEF1 ChIP on sorted thymic ST1/ST2 Vα14Tg iNKT cells, followed by qPCR using primers spanning a TCF1/LEF1-binding site upstream of the Gata3b promoter, the Rorc promoter, or an unrelated sequence at the β-globin gene locus. Numbers indicate mean fold enrichment of the immunoprecipitated DNA relative to the β-globin locus from three independent experiments is shown. Error bars represent SEM. *, P < 0.05; **, P < 0.01.