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. 2015 Apr 29;8:43. doi: 10.1186/s13045-015-0140-6

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© Dong et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The GAS5 expression in lung adenocarcinoma cell line and the effect of gefitinib combined with GAS5 on the proliferation of A549 cell line (A) qRT-PCR results demonstrating GAS5 expression in NSCLC cell lines (A549, H1650, H1975, HCC827) compared to human bronchial epithelial cells (HBEs). (B) IC₅₀ of four NSCLC cell lines. (C) GAS5 expression, measured by qRT-PCR, following the treatment of A549 cell line with pCDNA-GAS5 or empty vector. (D) The effect of GAS5 plus gefitinib on A549 proliferation in vitro. MTT assay was performed to determine the proliferation of pCDNA-GAS5- or empty vector-transfected A549 cells plus gefitinib.