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A colony-forming growth assay was performed to determine the proliferation of pCDNA-GAS5- or empty vector-transfected A549 cells plus gefitinib. The colonies were counted and captured. (A) Representative colony formation assay results of four groups. (B) GAS5 and gefitinib together synergistically suppress colony formation. The data represent the mean ± SD from three independent experiments. **P < 0.01
