Table 1.
Plasmids and strains used in the present study
| Plasmids or E. coli strain | Relevant properties or genetic marker | Source or reference |
|---|---|---|
| Plasmids | ||
| pACYCDuet | P15A ori, Cmr | Novagen |
| pCDFDuet | CloDE13 ori, Strr | Novagen |
| pETDuet | f1 ori, Ampr | Novagen |
| pGEX 5X-3 | pBR322ori, Ampr | GE Healthcare |
| pA-SeTAL | pACYCDuet carrying TAL from Saccharothrix espanaensis | Kim et al. (2013) [26] |
| pA-aorG-SeTAL-tyrA | pACYCDuet carrying TAL from S. espanaensis, aroG and tyrA from E. coli | Kim et al. (2013) [26] |
| pA-aorGfbr-SeTAL-tyrAfbr | pACYCDuet carrying TAL from S. espanaensis, aroG fbr , and tyrA fbr from E. coli | This study |
| pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr | pACYCDuet carrying TAL from S. espanaensis, aroG fbr , PPSA, tktA, and tyrA fbr from E. coli | Kim et al. (2013) [26] |
| pE-pIbF6′H2-pOs4CL | pETDuet harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. Each gene is controlled by independent T7 promoter. | This study |
| pE-pIbF6′H2-Os4CL | pETDuet harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. Each gene is controlled by one T7 promoter. | This study |
| pG-pIbF6′H2-pOs4CL | pGEX 5X-3 harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. F6′H2 is controlled by pTac promoter and 4CL is controlled by T7 promoter. | This study |
| pG-pIbF6′H2-Os4CL | pGEX 5X-3 harboring F6′H2 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. Each gene is controlled by one pTac promoter. | This study |
| pG-pIbF6′H1-Os4CL | pGEX 5X-3 harboring F6′H1 from Ipomoea batatas and 4CL from Oryza sativa. F6′H2 was fused with glutathione S-transferase. Each gene is controlled by one pTac promoter. | This study |
| BL21 (DE3) | F- ompT hsdS B(rB - mB -) gal dcm lon (DE3) | Novagen |
| BtyrR | BL21(DE3) ΔtyrR | Kim et al. (2013) [26] |
| BtyrR-tyrA | BL21(DE3) ΔtyrR/ΔtyrA | Kim et al. (2013) [26] |
| BydiI | BL21(DE3) ΔydiI | This study |
| BybgC | BL21(DE3) ΔybgC | This study |
| B-CM1 | BL21 harboring pE-pIbF6′H2-Os4CL | This study |
| B-CM2 | BL21 harboring pE-pIbF6′H2-pOs4CL | This study |
| B-CM3 | BL21 harboring pG-pIbF6′H2-pOs4CL | This study |
| B-CM4 | BL21 harboring pG-pIbF6′H2-Os4CL | This study |
| B-CM5 | BL21 harboring pE-pIbF6′H1-Os4CL | |
| B-CM6 | BL21 harboring pG-pIbF6′H2-Os4CL and pA-SeTAL | This study |
| B-CM7 | BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA | This study |
| B-CM8 | BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroGfbr-SeTAL-tyrAfbr | This study |
| B-CM9 | BL21 harboring pG-pIbF6′H2-Os4CL and pA-aroGfbr-ppsA-tktA-SeTAL-tyrAfbr | This study |
| B-CM10 | BydiI harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA | This study |
| B-CM11 | BybgC harboring pG-pIbF6′H2-Os4CL and pA-aroG-SeTAL-tyrA | This study |
| B-CM12 | BybgC harboring pG-pIbF6′H1-Os4CL, pA-aroGfbr-SeTAL-tyrAfbr, and pC-Sam5 | This study |