Table 1.
Physical characteristics an anti-HIV activity of griffithsin tandemers
| Name | Number of domains | Linker | Molecular Weight (kDa) | Anti-HIV EC 50 1 (pM) | Terminal binding surface distance (Å) |
|---|---|---|---|---|---|
| GRFT | 2 | none | 25.6 | 13.8 ± 0.3 | 50 |
| mGRFT (1GS-S [30]) | 1 | none | 12.8 | 119,300 ± 19,300 | n.a. |
| 2mGRFT | 2 | Gly-Thr-Gly | 25.8 | 2.7 ± 2.7 | 70 |
| 2mGRFT3 | 2 | (Gly-Thr-Gly) 3 | 26.2 | 2.6 ± 5.3 | 90 |
| 3mGRFT | 3 | Gly-Thr-Gly | 38.8 | 1.0 ± 2.9 | 90 |
| 4mGRFT | 4 | Gly-Thr-Gly | 51.8 | 1.2 ± 0.3 | 100 |
1Effective concentration at which 50% of CEM-SS cells are protected from the cytopathic effects of HIV-1RF infection.