Skip to main content
. 2015 Apr 28;3:e904. doi: 10.7717/peerj.904

Figure 1. Schematic of the translational switch using the amber codon-specific IY.

Figure 1

An amber stop codon is inserted next to the ATG translational start codon in the target gene (egfp). MJR1 is an amber suppressor tRNA. IYRS is an aminoacyl-tRNA synthetase that orthogonally recognizes IY and MJR1. Extracellular IY is taken up by the bacteria. The addition of IY in the media results in amber stop codon read-through and translation of the target gene. Translation is interrupted in the absence of IY. RF1, peptide chain release factor 1.