Table 1. Summary of alternative splicing assay results.
All genes except AT2G19480 were confirmed through PCR to have differential splicing patterns in pollen versus leaf sample types. Table indicates whether AS affected predicted protein, and if putative alternative transcriptional start sites (Alt. TSS) were identified.
| Locus | Annotation | AS affects protein | Putative Alt. TSS |
|---|---|---|---|
| AT1G09140 | At-SR30, involved in splicing | Y | N |
| AT5G52040 | At-RS41, involved in splicing | Y | N |
| AT5G65685 | Starch synthase | Y | N |
| AT4G21720 | Unknown | Y | N |
| AT3G17120 | Unknown | N | Y |
| AT3G06330 | Zn-finger, RING-CH-type | N | Y |
| AT3G58510 | RH11, RNA helicase | N | Y |
| AT1G67480 | Galactose oxidase/kelch repeat | N | Y |
| AT2G19480 | Nucleosome assembly protein | Y | N |
Notes.
- P
- pollen
- L
- leaves
- N
- no
- Y
- yes