Figure 8.

Implementation of image segmentation for all NBs in an AFM image. (a) Mask image obtained using the threshold method. The boundaries of the mask areas were taken as the initial guesses for boundary detection. (b) Initial contours (blue) and converged contours (green) for individual NBs. (c,d) Comparison of covered areas and volumes detected with the threshold method and the proposed method. One can see that both the covered areas and volumes detected with the threshold method are underestimated compared with that obtained by the proposed method.