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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: ChemMedChem. 2014 Oct 21;10(1):107–115. doi: 10.1002/cmdc.201402358

Table 3.

Apparent permeability blood brain barrier coefficient for inhibitors 3 and 4 in in vitro model.

Compound Initial luminal tracer concentration (μM) Final abluminal tracer concentration (μM) Papp (10−6 cm/s)
GRL-04810 100 3.33 ±0.70 50.38 ± 10.61
GRL-05010 100 4.01 ± 0.27 60.84 ± 3.97
DRV 100 0.62 ± 0.15 9.32 ± 2.25

In the in vitro model using a triple co-culture of rat astrocytes, pericytes and monkey endothelial cells, GRL-04810, GRL-05010, DRV (all 100 μM) were added to the luminal interface (termed blood side) of duplicate wells. The mathematical formula used for the calculation of Papp is described in Materials and Methods. Results show average values ±1 S.D. of duplicated determinations.