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. 2015 May 5;6:292. doi: 10.3389/fpls.2015.00292

Figure 4.

Figure 4

phyB is involved in regulating RL-induced PCD associated with ROS and SA production. (A) Trypan blue staining of four-week-old leaves of WT, phyB mutant, and phyB-ox-YFP under control, RL, and DCMU + RL treatment. (B) Areas of TB-stained foliar tissues in WT, phyB mutant, and phyB-ox-YFPas described in (A). (C) Relative ion leakage in WT, phyB mutant, and phyB-ox-YFP after RL or DCMU + RL. Asterisks indicate significant differences between the wild type and the mutants (Student's paired t-test: *P < 0.05, **P < 0.01). (D) H2DCFDA fluorescence (green) indicates ROS in leaves of WT, phyB mutant, and phyB-ox-YFP exposed to control, RL for 6 h, and DCMU + RL. (E) Measurement of ROS levels of WT, phyB mutant, and phyB-ox-YFP under control, RL, DCMU, and DCMU + RL conditions. (F) Detection of SA levels. Different letters indicate statistically significant differences between treatments (Duncan's multiple range test: P < 0.05). Values represent means ± SD of three independent replicates.