Table 1.
The Proteins with significant phosphorylation level changes under D, H, and DH.
| Protein group accessions | Protein name | Sequence | PhosphoRS site probabilities (only listed >75%) | Ratio of phosphorylation level | P-Value | ||||
|---|---|---|---|---|---|---|---|---|---|
| D/CK | H/CK | DH/CK | D/CK | H/CK | DH/CK | ||||
| B4F7W7 | MYB-CC type transfactor | tEPStPAPAsESPtQVGASNR | T(14): 97.1 | 2.40 | 2.90 | 3.62 | 0.0046 | 0.0002 | 0.0001 |
| B4FAB7 | CBS domain containing protein | nmESTSPSAASSSSTQLsPR | S(18): 100.0 | 3.57 | 2.81 | 2.43 | 0.0000 | 0.0003 | 0.0090 |
| B4FC96 | Zn-containing protein | gsPmPVSSPWsGGALAENNDNIASR | S(2): 99.6 | 0.59 | 0.54 | 0.53 | 0.0106 | 0.0318 | 0.0401 |
| B4FGQ4 | Protein kinase superfamily protein | tSSTsDSGR | S(5): 75.6 | 0.38 | 0.26 | 0.50 | 0.0000 | 0.0000 | 0.0259 |
| B4FI93 | Zinc finger ccch domain-containing protein 19-like | qNsGNER | S(3): 100.0 | 2.62 | 2.88 | 2.52 | 0.0019 | 0.0002 | 0.0066 |
| B4FKH3 | Unknown | kVEcLsGSPPMAISyVsk | S(17): 97.3 | 0.48 | 2.49 | 2.10 | 0.0004 | 0.0013 | 0.0293 |
| B4FKM0 | Protochlorophyllide reductase b | aQTAAVsSPSVTPASPSGk | S(7): 81.2 | 0.471 | 0.47 | 0.368 | 0.0002 | 0.0072 | 0.0013 |
| B4FQU2 | Translation initiation factor eif-2b beta subunit | tASStGDSDGk | T(5): 94.8 | 0.63 | 1.86 | 4.18 | 0.0245 | 0.0266 | 0.0000 |
| B4FRF2 | Oxygen-evolving enhancer protein 1 | gGsTGYDNAVALPAGGR | S(3): 96.7 | 2.30 | 2.06 | 1.62 | 0.0073 | 0.0104 | 0.1577 |
| B4FRX1 | Splicing arginine serine-rich 7 | gGSPTGsksP | S(3): 100.0; S(9): 100.0 | 1.59 | 1.90 | 2.54 | 0.1348 | 0.0220 | 0.0061 |
| B4FUV7 | Uncharacterized protein | sLscAER | S(3): 100.0 | 0.61 | 0.24 | 0.19 | 0.0148 | 0.0000 | 0.0000 |
| B4FY17 | Phospholipase c | sGSGNIAELPDQGsLR | S(14): 100.0 | 3.37 | 2.73 | 3.35 | 0.0001 | 0.0004 | 0.0004 |
| B4G250 | Heat shock protein 3 | sIQIsG | S(5): 100.0 | 0.64 | 9.30 | 17.34 | 0.0303 | 0.0000 | 0.0000 |
| B6SRZ6 | Ubiquitin-protein ligase | tVsAVGGGGGAPcsPSSR | S(3): 95.3; S(14): 100.0; | 2.55 | 3.33 | 2.26 | 0.0025 | 0.0000 | 0.0164 |
| B6SS42 | Reticulon-like protein b8-like | lFGGGEsR | S(7): 100.0 | 1.74 | 1.80 | 2.46 | 0.0753 | 0.0351 | 0.0079 |
| B6T1H0 | tpa: 40s ribosomal protein s9 | sEsLAk | S(3): 100.0 | 2.26 | 0.16 | 0.17 | 0.0084 | 0.0000 | 0.0000 |
| B6T1H0 | tpa: 40s ribosomal protein s9 | aSAAtSA | T(5): 80.0 | 1.96 | 0.20 | 0.26 | 0.0294 | 0.0000 | 0.0000 |
| B6TDN3 | Uncharacterized protein LOC100276536 | gAsAPPsPAGSPDGLIAALR | S(3): 100.0; S(7): 99.8 | 4.71 | 3.77 | 3.65 | 0.0000 | 0.0000 | 0.0001 |
| B6TH05 | Desi-like protein hag1-like | nQASPEsPGNNQNR | S(7): 99.8 | 5.19 | 2.84 | 3.67 | 0.0000 | 0.0002 | 0.0001 |
| B6U114 | Probable adp-ribosylation factor gtpase-activating protein agd14-like | tISSASsIGsAEGTSEQTk | T(1): 80.6 | 1.99 | 1.66 | 2.07 | 0.0259 | 0.0701 | 0.0328 |
| B6U0R6 | tpa: duf827 domain containing family protein | nEAsVEDLQGmEDVSLSLEEYsELAAk | S(4): 76.0 | 0.34 | 0.44 | 0.43 | 0.0000 | 0.0036 | 0.0070 |
| B6U4K3 | Vacuolar-sorting receptor 3-like | sLGLDVk | S(1): 100.0 | 0.61 | 0.44 | 0.54 | 0.0152 | 0.0041 | 0.0457 |
| C0HE61 | U2 snrnp-associated surp motif-containing | tsSSSER | S(2): 99.9 | 4.82 | 1.95 | 4.97 | 0.0000 | 0.0171 | 0.0000 |
| C0HIM6 | Integrin-linked protein kinase family protein | qLssGAAR | S(3): 100.0; S(4): 100.0 | 1.71 | 1.80 | 1.52 | 0.0851 | 0.0351 | 0.2165 |
| B8A0K2 | Calmodulin-binding family protein | tLsGGLQsPR | S(3): 100.0; S(8): 100.0 | 2.38 | 1.64 | 1.97 | 0.0051 | 0.0744 | 0.0465 |
| C0P3W9 | Phosphoenolpyruvate carboxykinase | sAPStPKR | S(1): 97.9; S(4): 2.2; T(5): 99.9 | 0.53 | 0.23 | 0.15 | 0.0019 | 0.0000 | 0.0000 |
| C0P3W9 | Phosphoenolpyruvate carboxykinase | sAPStPkRsAPTtPIk | S(1): 100.0; S(4): 94.4; T(5): 94.4; T(13): 99.8 | 0.22 | 0.22 | 0.21 | 0.0000 | 0.0000 | 0.0000 |
| C0P3W9 | Phosphoenolpyruvate carboxykinase | sAPSTPk | S(1): 100.0 | 0.58 | 0.41 | 0.29 | 0.0019 | 0.0000 | 0.0000 |
| C0P3W9 | Phosphoenolpyruvate carboxykinase | gEAAAQGAPstPR | S(10): 100.0; T(11): 100.0 | 0.64 | 0.59 | 0.61 | 0.0292 | 0.0664 | 0.1062 |
| C0PD30 | Fructose-bisphosphate aldolase | lAsIGLENTEANR | S(3): 100.0 | 3.00 | 3.33 | 2.67 | 0.0004 | 0.0000 | 0.0039 |
| C4J042 | Unknown | ymFAQItcGNGNAPtDSMk | T(7): 90.6 | 0.50 | 0.35 | 0.39 | 0.0007 | 0.0002 | 0.0022 |
| C4J1A8 | Probable protein phosphatase 2c 30-like | eQSsPTSNLsPR | S(4): 99.7; S(10): 99.8 | 12.67 | 2.23 | 8.77 | 0.0000 | 0.0044 | 0.0000 |
| C4J4L7 | Uncharacterized protein LOC100501611 | nsAGALHtSSSIPmSPTAk | T(8): 83.8 | 2.49 | 2.78 | 2.03 | 0.0032 | 0.0003 | 0.0369 |
| C4JBR4 | Glycine-rich protein 2 | sYGGsWGGGR | S(5): 100.0 | 2.23 | 1.76 | 2.25 | 0.0097 | 0.0423 | 0.0168 |
| F1DJV0 | Bzip transcription factor superfamily protein | nNLTEGGAEsDEEIR | S(10): 100.0 | 2.41 | 2.34 | 4.05 | 0.0044 | 0.0026 | 0.0000 |
| K7TNK3 | Leucine-rich repeat-containing protein ddb_g0290503-like | qItLSEIk | T(3): 100.0 | 1.60 | 1.94 | 2.20 | 0.1332 | 0.0181 | 0.0201 |
| K7TZQ1 | Serine threonine-protein kinase wnk4-like | qYPAsAGsSPSR | S(5): 93.4 | 2.71 | 2.20 | 3.81 | 0.0013 | 0.0050 | 0.0001 |
| K7U162 | RNA polymerase ii-associated factor 1 homolog | vEDIDQySEEYsE | S(12): 96.7 | 0.24 | 0.44 | 0.37 | 0.0000 | 0.0041 | 0.0013 |
| K7U573 | Nucleic acid binding protein | nQsNVNR | S(3): 100.0 | 2.70 | 2.20 | 2.82 | 0.0013 | 0.0052 | 0.0022 |
| K7U5U1 | Hypothetical protein ZEAMMB73_848936 | nAPsLGmGMMIk | S(4): 100.0 | 2.21 | 2.43 | 1.71 | 0.0106 | 0.0017 | 0.1138 |
| K7UF98 | Protein kinase superfamily protein | gAVAPAVDPsSPSSR | S(10): 78.0 | 2.25 | 1.51 | 1.78 | 0.0089 | 0.1336 | 0.0914 |
| K7V0H0 | Probable -trehalose-phosphate synthase | vmsVASPASPTsPSPPAPPR | S(3): 88.1 | 1.96 | 1.87 | 1.52 | 0.0304 | 0.0255 | 0.2158 |
| K7UKJ5 | Splicing arginine serine-rich factor 7 | sPSYNR | S(1): 100.0 | 2.11 | 3.50 | 4.67 | 0.0161 | 0.0000 | 0.0000 |
| K7VBI0 | Hypothetical protein | sIsETTLER | S(3): 100.0 | 3.40 | 0.49 | 1.82 | 0.0001 | 0.0129 | 0.0778 |
| K7VBA5 | hypothetical protein | aNsGGQPQAR | S(3): 100.0 | 1.77 | 3.12 | 3.91 | 0.0666 | 0.0001 | 0.0001 |
| Q8W149 | Cell division cycle 5-like | eIQTPNPmATPLAsPGPGItPR | S(14): 94.0; T(20): 94.0 | 5.15 | 2.94 | 3.58 | 0.0000 | 0.0001 | 0.0002 |
| Q9LLI8 | Probable cellulose synthase a catalytic subunit 2 | lTSGQQISGEIPDAsPDR | S(15): 99.9 | 0.30 | 0.41 | 0.31 | 0.0000 | 0.0016 | 0.0002 |
CK, control; D, drought stress; H, heat stress; DH, combined drought and heat stress. Each ratio was the average of three replicates.