Table 1.

Details for genotyping MTHFR and FVL genes.

Gene	SNP location	rs no.	Amino acid substitution	Forward primer	Reverse primer	Fragment	Annealing temperature	Enzyme
MTHFR	Exon 5	rs1801133	Ala 222 Val	TGAAGGAGAAGGTGT CTGCGGGA	GGACGGTGCGGTG AGAGTG	198 bp	68°C	HinfI
FVL	Exon 10	rs6020	Arg 506 Gln	TCAGGCAGGAACAA CACCAT	GGTTACTTCAAGGACAAAA TACCTGTAAAGCT	241 bp	56°C	HindIII