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. 2015 May 5;7:69. doi: 10.3389/fnagi.2015.00069

Figure 1.

Figure 1

miRNAs expression profile in diaphragm muscle of young mdx mice. (A) qRT-PCR analysis for the expression of miRNAs classified as degenerative miRNAs (Greco et al., 2009) (miR-1, miR-29c, miR-29b and miR-135a) performed on diaphragm muscles from wild-type (WT) and mdx mice at 4 weeks of age. All miRNAs were significantly down-regulated in dystrophic diaphragm of young mdx mice compared to WT. **p < 0.005, *p < 0.05. (B–F) qRT-PCR analysis for the expression of miRNAs classified as regeneration miRNAs (Greco et al., 2009), such as miR-31 (B), miR-34c (C), miR-206 (D), miR-449 (E) and miR-335 (F), in diaphragm of 4-week old WT and mdx mice. (G,H) qRT-PCR analysis for the expression of miRNAs classified as inflammatory miRNAs (Greco et al., 2009), namely miR-222 (G) and miR-223 (H) in diaphragm muscles from WT and mdx mice at 4 weeks of age. For all graphs, relative expressions were normalized to U6 snRNA and shown with respect to WT set to a value of 1. Values represent mean ± SEM; n = 4–7 per group. p values using Student’s t test assuming two-tailed.