Figure 3.

mIGF-1 promotes maturation of the myogenic program in diaphragm muscle of mdx mice. (A–F) Expression of MyoD (A), Myogenin (B), MEF-2C (C), MRF4 (D), β-enolase (ENO3) (E), and MCK (F), evaluated by real time PCR analysis in diaphragms of indicated genotypes at 4 weeks of age. Relative expressions were normalized to HPRT1 and shown with respect to WT, set to a value of 1. Values represent mean ± SEM; n = 4–7 per group. *p < 0.05, **p < 0.005, ***p < 0.0005, ^#p < 0.05, ^##p < 0.005 using one way ANOVA. (G,H) Histograms show miR-24 (G) and miR-31 (H) expression, analyzed by qRT-PCR in diaphragm muscles from WT, mdx, and mdx/mIGF-1 mice at 4 weeks of age. Expression levels were normalized to U6 snRNA and shown with respect to WT set to value of 1. For all graphs, values represent mean ± SEM; n = 5–7 per group. p values using one way ANOVA.