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. 2015 May 5;6:369. doi: 10.3389/fmicb.2015.00369

Table 1.

Primers used in agr and SCCmec typing by multiplex PCR.

Gene Primer Sequence (5′–3′) Amplicon size (bp) Reference
agrI agr I-F
agr I-R
ATGCACATGGTGCACATGC
GTCACAAGTACTATAAGCTGCGAT
441 Gilot et al. (2002)
agrII agr II-F
agr II-R
ATGCACATGGTGCACATGC
TATTACTAATTGAAAAGTGGCCATAGC
575 Gilot et al. (2002)
agrIII agr III-F
agr III-R
ATGCACATGGTGCACATGC
GTAATGTAATAGCTTGTAAAAAGTGGCCATAGC
323 Gilot et al. (2002)
agrIV agr IV-F
agr IV-R
ATGCACATGGTGCACATGC
CGATAATGCCGTAATACCCG
659 Gilot et al. (2002)
SCCmec I mec I-F
mec I-R
GCTTTAAAGAGTGTCGTTACAGG
GTTCTCTCATAGTATGACGTCC
613 Zhang et al. (2005)
SCCmec II mec II-F
mec II-R
CGTTGAAGATGATGAAGCG
CGAAATCAATGGTTAATGGACC
398 Zhang et al. (2005)
SCCmec III mec III-F
mec III-R
CCATATTGTGTACGATGCG
CCTTAGTTGTCGTAACAGATCG
280 Zhang et al. (2005)
SCCmec IVa mec IVa-F
mec IVa-R
GCCTTATTCGAAGAAACCG
CTACTCTTCTGAAAAGCGTCG
776 Zhang et al. (2005)
SCCmec V mec V-F
mec V-R
GAACATTGTTACTTAAATGAGCG
TGAAAGTTGTACCCTTGACACC
325 Zhang et al. (2005)
mecA mec 147-F
mec 147-R
GTGAAGATATACCAAGTGATT
ATGCGCTATAGATTGAAAGGAT
147 Zhang et al. (2005)

F, forward; R, reverse.