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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Alcohol Clin Exp Res. 2012 Apr 6;36(10):1688–1700. doi: 10.1111/j.1530-0277.2012.01791.x

Fig. 1.

Fig. 1

Experimental design. (A) A schematic of the experimental design. Animals were assigned to one of three treatment groups: control, chronic alcohol exposure, or withdrawal (with specific withdrawal time points covering the symptomatic period). The five time points selected for study are indicated, ranging from 4 to 48 hours after withdrawal. These were selected to capture early transcription changes as well as later changes associated with adaptation to withdrawal and the typical behavioral response after alcohol removal in the Lieber-DeCarli rat model. Control and chronic exposure animals were sacrificed at the same time as the withdrawal animal to account for differences in diurnal expression (ZT (Zeitgeber time) matched as indicated).