Fig. 1.—

The workflow for RNA-Seq and RFP. (A) The mixed parental strains or their hybrid were cultured in the YPD medium to the log phase. Cells were collected and treated by CHX for 2 min, and then divided into two aliquots, one for RNA-Seq and the other for RFP. (B) Diagram depicting the cis and trans effects for gene regulation. In the hybrid, genes face the same trans environment, so the divergence in gene regulation between alleles is only subject to the cis effect. Whereas in the parental strains, the divergence is subject to either trans or cis effect, or both. (C) Pearson correlation coefficient (r) among mRNA and RFP data between biological replicates and alleles (Sc: Saccharomyces cerevisiae, Sb: Saccharomyces bayanus). The asterisk indicates the hybrid strain.