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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Mitochondrion. 2010 Dec 13;11(2):342–350. doi: 10.1016/j.mito.2010.12.003

Fig. 2.

Fig. 2

Analysis of the frataxin (FXN) protein expression in isolated resting platelets (PLTs) and peripheral blood mononuclear cells (PBMCs) by western blot (A) and dipstick immunoassay (B). This figure shows that frataxin is clearly detected in mitochondria of both platelets and PBMCs of normal healthy donors. We also observed a dramatic decrease of this protein in mitochondria of both cell types from patients with both methods. Cytc, cytochrome c and GAPDH, glyceraldehyde 3-phosphate dehydrogenase, were used as a loading controls. 40 μg of protein were loaded in each lane and western blot analyses were performed in pair-wise manner (each FRDA sample is always processed with its matched NHV samples). The number below the patient identification represents the GAA repeat length of the corresponding patient as well as the treatment received at the time of the blood draw.