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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Mitochondrion. 2010 Dec 13;11(2):342–350. doi: 10.1016/j.mito.2010.12.003

Fig. 3.

Fig. 3

A. Purification of the mitochondria from peripheral blood mononuclear cells and assessment of their purity by western blot. Almost all of the mitochondrial proteins (DLAT, CoxVa, SOD2) are enriched in the mitochondrial pellet extract (MP) of mononuclear cells, while cytosolic proteins (LDH, GAPDH) are markedly decreased or absent in this extract. B. A representative 2-DE gel (IPG strip 3–10NL, 11 cm; 11% SDS-PAGE; Colloidal Coomassie blue staining) of mitochondrial proteins (200 ug) from PBMCs. Protein identification was performed using LC-QIT-MS/MS.