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. Author manuscript; available in PMC: 2015 Aug 10.
Published in final edited form as: J Control Release. 2015 Jan 7;205:134–143. doi: 10.1016/j.jconrel.2015.01.005

Fig. 2.

Fig. 2

The transduction efficiency of dE1/GFP/PEGbPHF depends on the PEGbPHF/Ad molar ratio at pH 7.4 and 6.4. (A) Fluorescence images of dE1/GFP or dE1/GFP/PEGbPHF complexes. Ad particles (1 × 1010 VP) were complexed with various molar ratios of PEGbPHF (5 × 104, 1 × 105, 5 × 105, or 1 × 106). U343, A549 or MCF7 cells were transduced for 30 min with naked dE1/GFP or dE1/GFP/PEGbPHF at pH 7.4 or 6.4. At 48 h after transduction, GFP images were observed under a fluorescence microscope. Original magnification: × 100. (B) Quantitative FACS analysis of GFP expression. The normalized transduction efficiency of dE1/GFP/PEGbPHF complexes relative to dE1/GFP was expressed as the fold increase relative to naked dE1/GFP. The data are presented as the means ± SD of triplicate experiments. Symbols: **P < 0.01, ***P < 0.001 versus dE1/GFP/PEGbPHF at pH 7.4.