Figure 4.
Anionic PAGE of proteins in parotid saliva from eight healthy subjects (50 μL per lane). One of the subjects in this pool (subject 5, Boston II) carried the PRP1/PRP3 RB variants. The position of the common aPRP isoforms were determined by reference to purified protein standards (not shown). The lower electrophoretic mobilities of the PRP1/PRP3 RB variants compared to PRP1/PRP3 is consistent with the substitution of the negatively charged Ser(Phos) residue at position 22 with a neutral a.a.