Figure 1.

Chromosomal location of Tn5 insertions. Seven mutant strains that were disrupted in genes important for capsule production, were identified from the screened Schu S4 library using the capsule mAbs 11B7 and 10E8. Rescue cloning was performed and the plasmids obtained were sent to The University of Iowa School of Medicine DNA Core for sequencing. A Tn5 primer was used to sequence the F. tularensis chromosomal DNA flanking the Tn5 transposon insertion. One end of the arrow shows the approximate site of transposon insertion into the chromosome and the arrow to the left or right shows the direction of the transposon element.