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Capsule and LPS O-antigen phenotypes of mutant strains. Bacteria were grown in MMH broth for ~24 h and lysed with Buffer Part A at 65°C. (A) The anti-capsule mAb (11B7) was used to detect capsule in whole cell lysates of wild type F. tularensis, O-antigen mutants, and complemented mutant strains by immunoblotting. (B) To detect LPS in immunoblots of whole cell lysates of wild type, mutants, and complemented mutants using the anti-O-antigen mAb (FB11).
