Figure 5.

Intracellular growth of wbtA, wzy, manB, 0846, hemH, dnaJ, waaY, and 0673/prsA intergenic mutants in MDMs. Triplicate wells containing human macrophages were infected for 1 h with unopsonized Schu S4 or mutant bacteria, washed to remove extracellular organisms, and then lysed with 1% saponin at 1, 16, and 24 h post infection to enumerate viable bacteria. Data presented show fold growth change from 1–16 h post-infection to 1–24 h post-infection. Data are from one experiment representative of three different experiments.