Table 1.
Murine virulence studies and experimentally determined intranasal LD50 values for F. tularensis Schu S4 and capsule and/or LPS mutants.
| Strain | Infection dose (CFU) | Survival ratio | Mean TTD | Calc LD50 (CFU) |
|---|---|---|---|---|
| INTRANASAL | ||||
| WT | 23 | 0/5 | 5 | <23 |
| dnaJ::Tn5 | 1.0 × 102 | 0/5 | 4.8 | <100 |
| dnaJ::Tn5 | 1.0 × 1.03 | 0/5 | 4.8 | |
| dnaJ::Tn5 | 1.0 × 104 | 0/5 | 4.4 | |
| dnaJ::Tn5 | 1.0 × 105 | 0/5 | 4 | |
| hemH::Tn5 | 1.1 × 102 | 0/5 | 6 | <110 |
| hemH::Tn5 | 1.1 × 103 | 0/5 | 5 | |
| hemH::Tn5 | 1.1 × 104 | 0/5 | 4.2 | |
| hemH::Tn5 | 1.1 × 105 | 0/5 | 4 | |
| manB::Tn5 | 1.0 × 102 | 5/5 | >27 | 5 × 103 |
| manB::Tn5 | 1.0 × 103 | 5/5 | >27 | |
| manB::Tn5 | 1.0 × 104 | 0/5 | 7.6 | |
| manB::Tn5 | 1.0 × 105 | 0/5 | 6.6 | |
| wbtA::Tn5 | 1.8 × 101 | 4/4 | >27 | 2.6 × 102 |
| wbtA::Tn5 | 1.8 × 102 | 3/5 | >27 | |
| wbtA::Tn5 | 1.8 × 103 | 0/5 | 17.6 | |
| wbtA::Tn5 | 1.8 × 104 | 0/5 | 7.6 | |
| wzy::Tn5 | 1.2 × 102 | 5/5 | >27 | 1.2 × 104 |
| wzy::Tn5 | 1.2 × 103 | 4/5 | 8, >27 | |
| wzy::Tn5 | 1.2 × 104 | 3/5 | 8, >27 | |
| wzy::Tn5 | 1.2 × 105 | 0/5 | 5.8 | |
| FTT0673plprsAp::Tn5 | 1.4 × 102 | 0/5 | 5.8 | <140 |
| FTT0673plprsAp::Tn5 | 1.4 × 103 | 0/5 | 5 | |
| FTT0673p/prsAp::Tn5 | 1.4 × 104 | 0/5 | 4.8 | |
| FTT0673plprsAp::Tn5 | 1.4 × 105 | 0/5 | 4.2 | |
| FTTOB46::Tn5 | 6.5 × 101 | 5/5 | >27 | 1.5 × 103 |
| FTTOB46::Tn5 | 6.5 × 102 | 4/5 | 25, >27 | |
| FTTOB46::Tn5 | 6.5 × 103 | 0/5 | 17 | |
| FTTOB46::Tn5 | 6.5 × 104 | 0/5 | 12 | |