Fig. 5.

Thermal stability of LmCld. ECD spectra of 12 μM holo-LmCld at (A) pH 7.0 (50 mM phosphate buffer), (B) pH 10.0 (50 mM glycine buffer), and (C) pH 4.0 (50 mM citrate/phosphate buffer), at 20 °C (black lines), after heating to 85 °C (red lines), and after cooling again to 20 °C (green lines). (D) Temperature mediated unfolding of 12 μM holo-LmCld followed at 222 nm at pH 4.0 (black), pH 7.0 (red), and pH 10.0. (E) DSC thermograms of 35 μM apo-LmCld (black) and 35 μM holo-LmCld (red) in 50 mM phosphate buffer, pH 7.0. The inset depicts UV–vis spectra of samples used in the DSC experiment; apo-LmCld (black), holo-LmCld (red). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)