Fig. 6.

Reversibility of heme binding and dissociation. UV–vis spectral transitions when heating (A) from 25 °C and 70 °C and cooling from 70 °C to 25 °C (C) of 4 μM holo-LmCld. Plot of the Reinheitzahl (B) and A_Soret/A₃₈₀ ratio (D) during heating (black dots and labels) and cooling (gray dots and labels). (E) ECD spectra of 6 μM holo-LmCld at 20 °C (black), 90 °C (red), and 20 °C after cooling (green); (F) Temperature mediated release of the prosthetic group followed at the Soret minimum at 414 nm (black line) with its sigmoid fit (red line). Conditions: 100 mM phosphate buffer, pH 7.0. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)