Table 1.
Strains and Plasmids used in this work
| Strain/Plasmid | Relevant Characteristics | References |
|---|---|---|
|
| ||
| HB101 | E. coli strain used for cloning | Stratagene |
| EC06 | E. coli ccmABCDEFGH::kan strain used for gene expression | [37] |
| pCS3O8 | = pACCyc3p: pLysS (Novagen®) derivative with T7 lysozyme gene replaced by expression cassette containing T7 promoter/lac operator system and 6xHis-tag sequence fused CYC3 from S. cerevisiae, ClmR; CCHL | [30] |
| pCS315 | pCS308 derivative with T7 promoter region replaced by tac promoter region of pMal-c2 (New England BioLabs®,Inc), ClmR | This work |
| pCS905 | pET-3a (Novagen®) derivative with T7 promoter region replaced by tac promoter region of pMal-c2 (New England BioLabs®,Inc.), AmpR | [33] |
| pCS13O3 | pCS905 derivative containing a decahistidine sequence-fused GFP gene variant, AmpR | [45] |
| pCS703 | = pSCyc7p: pET-3a (Novagen®) derivative containing Strep-tag II (IBA®) sequence-fused CYC7 from S. cerevisiae, AmpR | [30] |
| pCS1000 | Strep-tag II (IBA) sequence-fused CYC7 from pCS703 cloned XbaI and BamHI into pCS1303, replacing 10xHis::GFP, AmpR; Cyc7p | This work |
| pCS1011 | pCS1000 derivative with CYC7 encoding the amino acid sequence C-terminal of its CXXCH heme binding site replaced by R. capsulatus cycA to generate a fusion of Cyc7p and CycA joined at Cyc7p His 18, AmpR ; Cyc7pH18_sl9CycA | This work |
| pCS1736 | pCS1000 derivative, like pCS1011 but Cyc7p and CycA are joined N-terminally of the CXXCH motif of CycA at Cys14, AmpR; Cyc7pR13_C14CycA | This work |
| pCS1769 | pCS1011 derivative, deletion of the codons encoding thirteen amino-terminal amino acid residues (Ala-9 to Lys4) of the Cyc7p portion AmpR; K5Cyc7pH18_Sl9CycA | This work |
| pCS1302 | pCS905 derivative, Strep-tag II (IBA) sequence-fused GFP gene variant expressed under control of tac promoter/lac operator system, AmpR | [36] |
| pCS1726 | pCS1302 derivative containing Strep-tag II (IBA) sequence-fused cycA (matured cyt c2 gene variant) from R. capsulatus CycA, AmpR; CycA | [36] |
| pCS1730 | pCS1726 derivative with a codon for a Lys inserted at position ll,AmpR; CycA-insK11 | This work |
| pCS1760 | pCS1726 derivative with the Glu9 codon exchanged to a Leu codon, AmpR; CycA-E9L | This work |
| pCS1748 | pCS1730 derivative with the Glu9 codon exchanged to a Leu codon, AmpR; CycA-insK11+E9L | This work |
| pCS1754 | pCS1730 derivative with the Asn12 codon exchanged to a Thr codon, AmpR; CycA-insK11+N12T | This work |