Figure 1.
DOT1L inhibition impairs proliferation of MLL-PTD leukemia cell lines. (A) Growth of KOPM-88, EOL-1, MUTZ-11, MOLM-13 and HL-60 cells exposed to 10 μM EPZ004777. Viable cells were counted and re-plated at equal cell numbers in fresh media with fresh compound every 3–4 days. Results were plotted as percentage of split-adjusted viable cells in the presence of 10 μM EPZ004777 compared with DMSO vehicle control. (B) IC50 values for each cell line treated were determined graphically. Presence of MLL-PTD, MLL-AF9 translocation (MLL-AF9), or wild-type for MLL (MLL-wt) is indicated.