Fig 4. apoB48-LP antagonize agr-signaling and inhibit morbidity in an air-pouch model of S. aureus SSTI.

AH1677 (3x10⁷ CFUs) and apoB48-LP (100 nM) or vehicle control, were injected into dorsal air-pouches of 4APP-treated, Nox2 ^-/- mice. At 4 hrs post-infection, the mice were given a second dose of apoB48-LP or vehicle control. Twenty-four hrs post-infection, the following were determined: (A) percent weight loss; (B) clinical morbidity score (see Materials and Methods for details); (C) agr::P3 promoter activation in pouch lavage; and (D) bacterial burden in the pouch lavage and spleen. Results are the mean ± SEM of N = 7 mice/group from two independent experiments (A,B) and N = 3 mice/group (C,D). (E) Growth curves of USA300 isolate LAC grown in broth or broth with 10% serum from 4APP-treated, Nox2 ^-/- mice ± 100 nM apoB48-LP. Data shown are mean ± SEM from at least 2 independent experiments performed in duplicate. ns, not significant; **, p<0.01; ****, p≤0.0001.