Table 2. Primer sequence, GC % and annealing temperature of IL10 exons.

Primer	Sequence	GC%	Tm	Annealing temperature
IL10_1F	GGTTAGAGAAGGAGGAGCTCTAAGCA	50	60
IL10_1R	GGCGCAGGAGGAGGGTTCTT	65	58	60
IL10_2F	GGGCATCAAAAAGACCGCATTTCAGT	46	58
IL10_2R	TGTCCCTGCTGGTCTGTAGGA	57	56	58
IL10_3 & 4F	TCCCAGGGCCATGGAAGCAG	65	58
IL10_3 & 4R	TGCACGTGTGGGTTCAGCCT	60	56	58
IL10_5AF	TCCCAGCGTGAGGGAGAACA	60	56
IL10_5AR	GCGCCCGGCCTAGAACCAAA	65	58	58
IL10_5BF	GTTGAGCTGTTTTCCCTGACCTCCC	56	61
IL10_5BR	GTCAGACAAGAGTCAACTGACACCAGA	48	60	61
IL10_5CF	CCTAAATTTGGTTCTAGGCCGGGCG	56	61
IL10_5CR	TAGGGGGTAGCTGGCTTCCTTTCTC	56	61	61