Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Aug 13;1:14034. doi: 10.1038/mtm.2014.34

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

PMC Copyright notice

rAAV9 stability testing. (a) Infectious titer assay: iodixanol-purified rAAV9-GFP was exposed to sodium citrate/phosphate buffers in the range of pH2.55–8.05, at room temperature for the time period of 2 hours, 24 hours, or 3 weeks. After incubation, an aliquot was diluted in Lactate Ringer solution and used to infect C12 cells coinfected with Ad5 (multiplicity of infection of 5). GFP (+) cells were visually scored at 48 hours after infection. (b) EM studies: viral capsid were visualized after treatment at different temperatures (rows) in buffers of different pHs (columns).