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. 2015 May 5;10(5):e0125553. doi: 10.1371/journal.pone.0125553

Fig 1. QRT-PCR analysis of untreated SI-NETs at different stages of disease to detect previously analyzed SI-NET tissue miRNAs.

Fig 1

Total RNA was isolated from serum samples of seven healthy donors (HD 1–7), seven primary SI-NET (PT 1–7), seven lymph node metastases (LNM 1–7) and seven liver metastases (LM 1–7). (A) MiR-96 was significantly higher in PT and LM patients vs HD, whereas miR-182, -196a and -200a levels were exclusively and significantly higher in LM patients vs HD. Conversely, miR-183 level was never significantly higher in any SI-NET patient group vs HD (B) MiR-31, -129-5p, -133a and -215 levels were significantly lower in untreated patients at different stages vs HD. Results were plotted using the 2-ΔΔCt method with miR-16 expression (set to 1) from each individual sample for normalization. Significance was calculated by One-Way ANOVA followed by Dunnett’s test. * = p < 0.05, ** = p < 0.01 and *** = p < 0.001.