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. 2015 May 5;10(5):e0125229. doi: 10.1371/journal.pone.0125229

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© 2015 Wu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (a) Protein purity was assessed via 12% SDS-PAGE. M, protein marker; lane 1, crude extracts of cells harboring pET-28b(+) after induction with IPTG; lane 2, crude extracts of cells harboring the recombinant plasmid after induction with IPTG; lane 3, purified protein. (b) Western blot analysis using the anti-6×His antibody as a probe. Lane 1, negative control, crude extracts of cells harboring pET-28b(+) with IPTG induction; lane 2, purified protein. (c) Molecular mass determination via gel filtration chromatography. The V_e of recombinant ClIDH was 10.7 mL.