Table 1. Kinetic parameters of wild-type and mutant ClIDH.

Enzyme	NAD+			NADP+
	K m (μM)	k cat (s-1)	k cat/K m (μM-1 s-1)	K m (μM)	k cat (s-1)	k cat/K m (μM-1 s-1)
ClIDH (Mg2+)	262.6±7.4	36.7	0.14	−	−	−
ClIDH (Mn2+)	309.1±11.2	84.7	0.27	−	−	−
D487R/L488H (Mn2+)	663.8±48.6	9.7	0.015	156.7±4.8	55.1	0.35