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. 2015 May 5;10(5):e0125910. doi: 10.1371/journal.pone.0125910

Fig 4. Flagellin activates PI3K/Akt/mTORC1 signaling via TLR5.

Fig 4

Ana-1 cells were lysed, and total proteins were extracted. A. the cells were stimulated at the indicated times with S. typhimurium flagellin (100 ng/ml). B. the cells were stimulated by flagellin (100 ng/ml) for 24 h in present or absent of TLR5 or TLR4 antibody. Total proteins were subjected to immunoblot with phospho-Akt(Ser473), phospho-mTOR (Ser2448), phospho-S6 (Ser240/244), and phospho-4EBP1 (Thr37/46). Blots were stripped and probed with total Akt, mTOR, S6, and 4EBP1 antibodies. β-actin was used as a loading control. Three separate experiments were performed. *p< 0.05, ns. not significant.