Fig 6. Rapamycin prevents flagellin-induced IκBα degradation and p65 and STAT3 activation.

Ana-1 cells were pretreated with rapamycin (100 nM) for 4 h and stimulated with 100 ng/ml flagellin for 24 h. Total proteins were separated by SDS-PAGE and probed with IκBα, phospho-NF-κB p65 (Ser536), and phospho-STAT3 (Tyr705) antibodies. The blots were stripped and probed with NF-κB p65 and STAT3 antibodies. β-actin was used as the loading control. Three separate experiments were performed.