Fig 8. Rapamycin downregulates flagellin-induced IL-6 expression in mouse peritoneal macrophages.

Mouse peritoneal macrophages were pretreated with rapamycin (100 nM) for 4 h before being stimulated with 100 ng/ml flagellin for 24 h. Supernatants were assayed for IL-6 by ELISA. flagellin-induced IL-6 expression is inhibited by rapamycin. Experiments were made in triplicate, and data are representative of three separate experiments (mean ± SD). *p< 0.05 compared with flagellin group.