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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Bone Marrow Transplant. 2013 Sep 30;49(4):469–476. doi: 10.1038/bmt.2013.152

Table 2.

Trials using lower DMSO concentrations in cryopreservation

DMSO concentration References Cryopreservation medium and comments
10% (2,5,13,16,18, 19,24,36,39,40,43,50,55–57,62,64,68,69,73,74)

  • Generally, 10% DMSO + plasma (concentration: 2–22.5%) in saline, culture media or electrolyte injection solutions (e.g., Normosol-R).
9% (12)
7.5% (8,64,104)

  • Donmez (8): 3% HES was added.
5% (3,7,64,67,108,110–113)

  • 5% DMSO instead of 10% DMSO could

    • decrease DMSO toxicity (109)

    • reduce the release of intracellular components from dead cells (109)

    • improve the kinetics of engraftment (108)

  • Hayakawa (110): 5% DMSO+6% pentastarch+25% human albumin, heparin and DNAse, which was equivalent to/better than the 10% DMSO medium.

  • Keung and Rowley (67,108): 6% HES + 5% DMSO
<5% (64,109,114)

  • Cell recovery rate may be reduced with reduced DMSO concentration

  • Other CPAs (e.g., HES, trehalose) are recommended to be combined.
*

HES: hydroxyethyl starch