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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Int J Cancer. 2010 Aug 15;127(4):780–790. doi: 10.1002/ijc.25011

Fig 2.

Fig 2

B.P. CM reduces colony formation of colon cancer cells or AOM-treated non-transformed colonocytes. (A) HT-29 colon cancer cells pretreated with B.P. CM for 2 weeks were plated on a soft agar and incubated for additional 2 weeks. At the end of the experiment, photographs were taken and the number of colonies was counted as described in materials and methods section. The data are shown as mean ± SD; * p<0.009 versus control. Bar, 200 µm. (B) Non-transformed colonic epithelial cells (NCM 460) pretreated with B.P. CM for 2 weeks, were plated on a soft agar with or without AOM (1 mg/ml) and incubated for 2 additional weeks. At the end of the experiment, photographs were taken and the number of colonies was counted. The data are shown as mean ± SD; * p<0.009 versus control.