Table 2. Short-listed candidate genes located in the PNR1 QTL on Chr 12.
| Index | Symbol | Gene | Microarray | Microarray | Literature Significance # |
|---|---|---|---|---|---|
| BM vs. PE | Blood vs. PE | ||||
| FC* | FC* | ||||
| 1 | Hif1a | Hypoxia inducible factor 1, alpha subunit | 4.72 | 7.43 | [29] |
| 2 | Prkch | Protein kinase C, eta | 2.20 | 2.37 | [23] |
| 3 | Fntb | Farnesyltransferase, CAAX box, beta | -1.91 | -3.63 | [22] |
| 4 | Dhrs7 | Dehydrogenase/reductase SDR family member 7 | -2.79 | [34] | |
| 5 | 1200003C05Rik | RIKEN cDNA 1200003C05 gene | -1.65 | -1.71 | |
| 6 | Ppm1a | Protein phosphatase 1A | -1.886 | [35] | |
| 7 | Snapc1 | snRNA-activating protein complex subunit 1 | 1.493 | 1.58 | |
| 8 | Max | Max protein | 1.79 | [36] | |
| 9 | Mnat1 | Menage a trois 1/ cyclin H assembly factor | -1.55 | ||
| 10 | Daam1 | Disheveled-associated activator of morphogenesis | [37] | ||
| 11 | Rhoj | Ras homolog family member J | [38] | ||
| 12 | Sgpp1 | Sphingosine-1-phosphate phosphatase 1 | [39] | ||
| 13 | Syne2 | Spectrin repeat containing, nuclear envelope 2; Nesp2g | [40] | ||
| 14 | Esr2 | Estrogen receptor 2 (beta) | [41] | ||
| 15 | Akap5 | A kinase (PRKA) anchor protein 5 | [42] | ||
| 16 | Gpx2 | Glutathione peroxidase 2 | [43] |
Sixteen genes were prioritized through a selective literature search, inclusion in 2 NR gene expression studies, and presence of SNPs differing in the parental strains.
(-) indicates mRNAs were downregulated as PMNs transit from BM to PE and Blood to PE. BM—Bone Marrow; PE—Peritoneum; FC—Fold Change.
* FC significant at p<0.05.
#Using PubMed, each candidate gene was searched using the “AND” function with 11 terms selected to be relevant to NR including “neutrophil”, “neutrophil recruitment”, “neutrophil activation”, “neutrophil infiltration”, “neutrophil chemotaxis”, “innate immunity”, “cell migration”, “actin”, “cytoskeleton”, and “GTPases”. Bolded genes show significance with both microarray data sets.