Figure 4.

NMR analysis of TpsB4 POTRA1 domain and TpsA4/CupB5 interactions. (A) ¹H-¹⁵N HSQC spectra of ¹⁵N-labelled TpsB4-P1 (black) with 20 molar equivalents of TpsA4^79–95 (red). (B) Expanded ¹H-¹⁵N HSQC spectra for ¹⁵N-labelled TpsB4-P1 with two molar equivalents of TpsA4^79–95 (red) or CupB5^80–96 (purple). (C) Two representative regions of ¹H-¹⁵N HSQC spectra for ¹⁵N-labelled TpsB4-P1 titrated against TpsA4^79–95. Examples of the corresponding binding curves are displayed below. (D) Composite TpsA4^79–95 and CupB5^80–96 interactions mapped onto the surface of TpsB4-P1. Red areas indicate residues in close vicinity to TpsA4^79–95 and CupB5^80–96. Numbering is based on mature proteins minus N-terminal signal sequences. An interactive view is available in the electronic version of the article